Validation of five-colony pool analysis using multiplex real-time PCR for detection of diarrheagenic Escherichia coli.

نویسندگان

  • F Barletta
  • T J Ochoa
  • L Ecker
  • A I Gil
  • C F Lanata
  • T G Cleary
چکیده

Five Escherichia coli colonies/patient were studied to evaluate the reliability of a multiplex real-time PCR assay for detection of diarrheagenic Escherichia coli groups, using a pool of five colonies rather than individual colonies. Sensitivity and specificity were 98% and 100%, respectively, at a fifth of the cost of the individual colony analysis.

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عنوان ژورنال:
  • Journal of clinical microbiology

دوره 47 6  شماره 

صفحات  -

تاریخ انتشار 2009